Journal: International Journal of Cosmetic Science

Article Title: Multidirectional activity of bakuchiol against cellular mechanisms of facial ageing ‐ Experimental evidence for a holistic treatment approach

doi: 10.1111/ics.12784

Figure Lengend Snippet: Antioxidative and anti‐inflammatory capacity of bakuchiol and retinol. (a) Antioxidative efficacy of bakuchiol (100 μM) or retinol (100 μM) compared to the high standard trolox (25 μM) and the solvent control determined by a DPPH antioxidant assay through absorbance measurement at 524 nm after 10, 30 and 60 min. N = 12. (b) Antioxidative power expressed in antioxidative units of bakuchiol, retinol or the high standard vitamin C (vit. C) determined using electron spin resonance spectroscopy. (c) ELISA‐based measurement of prostaglandin E2 (PGE2) levels in unstressed human dermal fibroblasts (HDFs), LPS‐stressed control HDFs, and in LPS‐stressed HDFs treated with the high standard diclofenac (25 ng/mL), bakuchiol or retinol (both applied at 1.25, 2.5, 5, 10 μM) for 24 h. N = 12. (d) Macrophage migration inhibitory factor (MIF) protein levels in unstressed HDFs, in HDFs stressed by a DPBS incubation and in stressed HDFs treated with bakuchiol or retinol (both applied at 1 and 10 μM) for 48 h determined by ELISA. N = 10. Results are depicted as mean ± SD. Statistics were performed by RM‐ANOVA with post‐hoc pairwise comparison based on Blom‐transformed ranks for Figure or by a pairwise Wilcoxon signed rank test for Figure . Significant differences are marked with an asterisk (** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Human dermal fibroblasts (HDFs) from multiple donors were obtained from Tissue Solutions Ltd (Glasgow, UK), Lonza (Basel, Switzerland), tebu‐bio (Heerhugowaard, Netherlands) or isolated from full‐thickness skin explants purchased from Alphenyx (Marseille, France) as described before [ ].

Techniques: Solvent, Control, Antioxidant Assay, Electron Paramagnetic Resonance, Spectroscopy, Enzyme-linked Immunosorbent Assay, Migration, Incubation, Comparison, Transformation Assay

Journal: International Journal of Cosmetic Science

Article Title: Multidirectional activity of bakuchiol against cellular mechanisms of facial ageing ‐ Experimental evidence for a holistic treatment approach

doi: 10.1111/ics.12784

Figure Lengend Snippet: Effects of bakuchiol and retinol on cell activity and major ECM components. (a) Fibroblast growth factor 7 (FGF7) protein levels in control HDFs and in HDFs treated with 10 μM bakuchiol or retinol for 24 h. N = 13. (b) Quantification of WST‐1 metabolization in control HDFs and in HDFs treated with 10% triton‐X, bakuchiol or retinol (both applied at 1 and 10 μM) for 72 h. Difference in absorbance at 620 and 450 nm is depicted. N = 12. (c) Collagen, type VII, alpha 1 (COL7A1) protein levels in control HDFs and in HDFs treated with the high standard TGF‐β (10 ng/mL) and sodium ascorbate (SA; 11 μg/mL), as well as with bakuchiol or retinol (4 h: 1 and 10 μM, extended incubation: 10 μM for both test substances) for 4 h or for an extended incubation time (72 h or 96 h depending on cell confluence). N = 10 for 4 h, n = 11 for extended incubation. (d) Collagen, type I, alpha 1 (COL1A1) protein levels in control HDFs and in HDFs treated with the high standard TGF‐β and SA as aforementioned, bakuchiol or retinol (both applied at 1 and 10 μM) for 4 h. N = 11. (e) Fibronectin (FN) protein levels in control HDFs and in HDFs treated with 10 μM bakuchiol or retinol for 24 h. N = 11. All protein levels were determined by ELISA. Results are depicted as mean ± SD. Statistics were performed by RM‐ANOVA with post‐hoc pairwise comparison for Figure or by a pairwise Wilcoxon signed rank test for Figure . Significant differences are marked with an asterisk (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Human dermal fibroblasts (HDFs) from multiple donors were obtained from Tissue Solutions Ltd (Glasgow, UK), Lonza (Basel, Switzerland), tebu‐bio (Heerhugowaard, Netherlands) or isolated from full‐thickness skin explants purchased from Alphenyx (Marseille, France) as described before [ ].

Techniques: Activity Assay, Control, Incubation, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Advanced Science

Article Title: A New Strategy to Inhibit Scar Formation by Accelerating Normal Healing Using Silicate Bioactive Materials

doi: 10.1002/advs.202407718

Figure Lengend Snippet: A) Differences in wound healing between infants and adults. In infants, when skin injury, fibroblasts activated spontaneously and rapidly migrated to the wound site, thereby accelerating wound healing, and inhibiting scar formation. Conversely, in adults, fibroblasts exhibit limited proliferation and migration activity, impeding wound healing and leading to scar formation. B) Accelerated normal wound healing by bioactive materials reduces the scar hyperplasia. Inspired by the scar‐free skin wound healing process in infants, a 45S5 bioglass/sodium alginate composite hydrogel (BG/SA) was used to accelerate normal wound healing through enhancing fibroblast migration by SiO 3 2− , thereby reducing scar formation.

Article Snippet: Hypertrophic scar fibroblasts (HSF, model number: HUM‐iCell‐s035) and human skin dermal fibroblasts (HDF, model number: HUM‐iCell‐s001) were purchased from iCell (Shanghai) Biotechnology Co., Ltd.

Techniques: Migration, Activity Assay